Below are the NEXUS lane hours of operation at ports that offer NEXUS service.
If you are taking any of the Niagara area bridges please double check this information at the Niagara Bridge Authority website.
Hours of Operation for NEXUS Lanes
| Location (Canada) | Province | Location (U.S.) | State | Hours of service into Canada | Hours of service into U.S. | |
| Abbotsford-Huntingdon | British Columbia | Sumas | Washington | The NEXUS lane is temporarily closed. NEXUS members whose travel is essential should use the regular primary inspection lanes to seek entry to Canada. | Monday to Friday 8 am to 11 am Monday to Friday Radio Frequency Identification (RFID)- ready including NEXUS 11 am to 6 pm Weekends Radio Frequency Identification (RFID)- ready including NEXUS 8 am to 5 pm | |
| Aldergrove | British Columbia | Lynden | Washington | The NEXUS lane is temporarily closed. NEXUS members whose travel is essential should use the regular primary inspection lanes to seek entry to Canada. | n/a | |
| Boundary Bay | British Columbia | Point Roberts | Washington | The NEXUS lane is temporarily closed. NEXUS members whose travel is essential should use the regular primary inspection lanes to seek entry to Canada. | Seven days a week 9 am to 7 pm | |
| Coutts | Alberta | Sweetgrass | Montana | The NEXUS lane is temporarily closed. NEXUS members whose travel is essential should use the regular primary inspection lanes to seek entry to Canada. | Seven days a week Until further notice, NEXUS is accepted in all lanes. | |
| Emerson | Manitoba | Pembina | North Dakota | The NEXUS lane is temporarily closed. NEXUS members whose travel is essential should use the regular primary inspection lanes to seek entry to Canada. | Seven days a week 6:30 am to 8 am Closed on holidays. | |
| Fort Erie (Peace Bridge) | Ontario | Buffalo (Peace Bridge) | New York | Monday to Friday The NEXUS lane will remain open until 10 pm when there is a Buffalo Sabres home game. | From June 7 Until Labour Day Monday to Friday 5 am to 11 pm and Weekends and holidays 6 am to 11 pm | |
| Fort Frances | Ontario | International Falls | Minnesota | The NEXUS lane is temporarily closed. NEXUS members whose travel is essential should use the regular primary inspection lanes to seek entry to Canada. | Seven days a week NEXUS is accepted in all lanes | |
| Lansdowne | Ontario | Alexandria Bay | New York | The NEXUS lane is temporarily closed. NEXUS members whose travel is essential should use the regular primary inspection lanes to seek entry to Canada. | Monday to Friday: 7 am to 5 pm Weekends: 10 am to 2 pm | |
| Niagara Falls (Queenston/Lewiston Bridge) | Ontario | Niagara Falls (Queenston/Lewiston Bridge) | New York | The NEXUS lane is temporarily closed. NEXUS members whose travel is essential should use the regular primary inspection lanes to seek entry to Canada. | Monday to Friday 6 am to 8 am (excluding holidays) | |
| Niagara Falls (Whirlpool-Rapids Bridge) | Ontario | Niagara Falls (Whirlpool-Rapids Bridge) | New York | Seven days a week 7 am to 11 pm | Seven days a week 7 am to 11 pm | |
| Pacific Highway | British Columbia | Blaine | Washington | Seven days a week 7 am to 7 pm | Seven days a week 6 am to 9 pm | |
| Sarnia (Blue Water Bridge) | Ontario | Port Huron (Blue Water Bridge) | Michigan | Seven days a week including holidays 7 am to 10 pm | Monday to Friday 6 am to 9 pm Weekends 8 am to 9 pm Holidays 8 am to 9 pm | |
| Sault Ste. Marie | Ontario | Sault Ste. Marie | Michigan | The NEXUS lane is temporarily closed due to construction. | Monday to Friday 7 am to 8 am | |
| St. Armand-Philipsburg | Quebec | Highgate Springs | Vermont | Monday to Friday 4 pm to 6 pm effective | Seven days a week 6:30 am to 8 am Friday 3 pm to 6 pm | |
| St. Bernard-de-Lacolle | Quebec | Champlain | New York | Monday to Friday 6 am to midnight effective September 7, 2021 | Seven days a week 6:00 am to 8 pm | |
| Stanstead | Quebec | Derby Line | Vermont | Monday to Friday 3 pm to 5 pm Closed on holidays. | N/A | |
| St. Stephen | New Brunswick | Calais | Maine | The NEXUS lane is temporarily closed. NEXUS members whose travel is essential should use the regular primary inspection lanes to seek entry to Canada. | Monday to Friday 8 am to 11 am (EST) | |
| Surrey (Douglas / Peace Arch) | British Columbia | Blaine (Douglas / Peace Arch) | Washington | Seven days a week 7 am to 7 pm | Seven days a week 6 am to midnight. | |
| Windsor (Ambassador Bridge) | Ontario | Detroit (Ambassador Bridge) | Michigan | Monday to Friday 6 am to midnight and Weekends and holidays 7 am to midnight | Monday to Friday 5:30 am to 8 pm and Weekends and holidays 11:30 am to 7 pm | |
| Windsor (Detroit-Windsor Tunnel) | Ontario | Detroit (Detroit-Windsor Tunnel) | Michigan | The NEXUS lane is temporarily closed. NEXUS members whose travel is essential should use the regular primary inspection lanes to seek entry to Canada. | Monday to Friday | |
| Woodstock | New Brunswick | Houlton | Maine | Monday to Friday 3 pm to 6 pm Closed on holidays. | Monday to Friday 7 am to 10 am (EST) |